RNA Preparation and Purification
New England Biolabs, Inc. NEBNext rRNA Depletion Kit (Bacteria) – 24 reactions
The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).
New England Biolabs, Inc. NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads – 24 reactions
The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic). For convenience, this kit includes RNA Clean beads.
Research Products International Corp Quick-RNA MiniPrep Kit w/Zymo-Spin IIICG Columns, Capped, & Spin-Away Filters, 50 Preps
The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
Sigma Aldrich Fine Chemicals Biosciences RNA from yeast
RNA from yeast is the total RNA obtained from Saccharomyces cerevisiae.
Research Products International Corp ZR-96 RNA Clean & Concentrator Kit, 2 x 96 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Epoch Life Science Inc GenCatch™ Viral RNA Miniprep Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify viral nucleic acids from cell-free samples such as plasma, serum, body fluid, urine, and cell culture supernatant. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature proteins. Purified viral RNA is suitable for applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of RNA from pathogenic viruses such as hepatitis C virus (HCV) for RT-PCR screening of clinical samples.
New England Biolabs, Inc. NEBNext Ultra™ II Directional RNA Library Prep with Sample Purification Beads – 24 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. For convenience, this kit includes SPRISelect beads.
Alkali Scientific TRAzol, RNA Purification Reagent Kit, 100ml, Store at 2-8°C, protect from light
TRAzol, RNA Purification Reagent Kit, 100ml TRAzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour. Key features of TRAzol Reagent include: • Permits the isolation of RNA, DNA, and protein from the same sample • Offers superior lysis capability, even with difficult sample types • Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria Reliably purify RNA from multiple sample volumes and sources TRAzol Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purific
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Brain, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Small Intestine: Jejunum, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Monkey (Cynomolgus) Normal Tissue: Brain, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.